Essential Oils of Artemisia herba-alba, Mentha pulegium, and Cedrus atlantica: Chemical compositions, in vitro, in vivo, in silico Antifungals Activities, and Genotoxicity

Imane Oualdi, Mohammed Merzouki, Sabir Ouahhoud, Khadija Chakrone, Redouane Benabbes, El Bekkaye Yousfi, Allal Challioui, Belkheir Hammouti, Rachid Touzani

Abstract


The extraction of three essential oils from medicinal and aromatic plants was carried out using steam distillation. Phytochemical analysis was performed using gas chromatography coupled with mass spectrometry (GC/MS). The results revealed the presence of several bioactive compounds: camphor, identified as the chemotype of the essential oil of Artemisia herba-alba (AhaEO); pulegone, the predominant compound in the essential oil of Mentha pulegium (MpEO); and β-himachalene, the chemotype of the essential oil of Cedrus atlantica (CaEO). The antifungal activity of these essential oils (EOs) was evaluated against Fusarium oxysporum albedinis (Foa) using the direct contact method on PDA medium and in soil. The essential oils exhibited significant antifungal effects. Furthermore, genotoxic effects were assessed, and the results showed that the application of essential oils at concentrations of 2 and 4 µg/mL did not induce DNA damage. Computational simulations and molecular interaction analyses were also performed to validate the experimental findings.

Keywords


Antifungal activity; Essential oils; Fusaruim Oxysporum albedinis; Genotoxicity

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References


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DOI: https://doi.org/10.17509/ajse.v5i1.80693

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