Comprehensive Characterization of Moroccan Honey Varieties (cedar, euphorbia, eucalyptus, carob, and thyme): Insights into Phytochemical and Physico-chemical Properties

Abdelilah Benmehdi, El Hassania Loukili, Mohamed Taibi, Aymane Allay, Abdellah Azougay, Bouchra El Guerrouj, Hanae Serghini-Caid, Rachid Sabbahi, Khalil Azzaoui, Rachid Salghi, Belkheir Hammouti, Mohammed Ramdani

Abstract


We analyzed five Moroccan honey varieties: cedar, euphorbia, eucalyptus, carob, and thyme, focusing on their physicochemical and phytochemical properties. Using gas chromatography-mass spectrometry, high-performance liquid chromatography, and X-ray fluorescence, we measured key parameters like pH, refractive index, moisture content, and Brix degree, comparing them to Codex Alimentarius standards. The chemical composition varied significantly between samples. We also evaluated antioxidant capacity through DPPH and total antioxidant assays. Methyl hepta-2,4-dienoate was predominant in euphorbia and eucalyptus honey, while 3-methoxy-2-methyl-cyclohex-2-enone was unique to carob honey. All samples contained polyphenols like caffeic acid and naringenin. Carob honey has high fructose and glucose levels and exceptional antioxidant capacity. These findings highlight the health-promoting potential of Moroccan kinds of honey for food and nutraceutical applications.

Keywords


Antioxidant capacity; Health-promoting potential; Moroccan honey; Physicochemical properties; Phytochemical profiles; Polyphenols

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References


Abu-Tarboush, H. M., Al-Kahtani, H. A., and El-Sarrage, M. S. (1993). Floral-type identification and quality evaluation of some honey types. Food Chemistry, 46(1), 13-17.

Alghamdi, B. A., Alshumrani, E. S., Saeed, M. S. B., Rawas, G. M., Alharthi, N. T., Baeshen, M. N., Helmi, N. M., Alam, M. Z. and Suhail, M. (2020). Analysis of sugar composition and pesticides using HPLC and GC–MS techniques in honey samples collected from Saudi Arabian markets. Saudi Journal of Biological Sciences, 27(12), 3720-3726.

Al-Hindi, R. R., and Shehata, A. (2014). Evaluation of antioxidant and antibacterial activities and essential elements content of locally produced honey in Saudi Arabia. Journal of Life Science, 11(5), 175-185.

Aljadi, A. and Ka maruddin, M. (2004). Evaluation of the phenolic contents and antioxidant capacities of two Malaysian floral honeys. Food chemistry, 85(4), 513-518.

Aljohar, H. I., Maher, H. M., Albaqami, J., Al-Mehaizie, M., Orfali, R., Orfali, R. and Alrubia, S. (2018). Physical and chemical screening of honey samples available in the Saudi market: An important aspect in the authentication process and quality assessment. Saudi Pharmaceutical Journal, 26(7), 932-942.

Alloune, R., Liazid, A. and Tazerout, M. (2012). Etudes comparatives de deux plantes oléagineuses locales pour la production du biodiesel en Algérie. Revue des Energies Renouvelables SIENR, 12, 19-22.

Alvarez-Suarez, J. M., Tulipani, S., Romandini, S., Bertoli, E. and Battino, M. (2010). Contribution of honey in nutrition and human health: a review. Mediterranean Journal of Nutrition and Metabolism, 3, 15-23.

Amarowicz, R., Troszyńska, A. and Shahidi, F. (2005). Antioxidant activity of almond seed extract and its fractions. Journal of Food Lipids, 12(4), 344-358.

Amri, A., Ladjama, A. and Tahar, A. (2008). Etude de quelques miels produits à l’est Algérien: aspect physico-chimique et biochimique. Synthese: Revue des Sciences et de la Technologie, 17, 57-63.

Ballabio, D., Robotti, E., Grisoni, F., Quasso, F., Bobba, M., Vercelli, S., Gosetti, F., Calabrese, G., Sangiorgi, E., Orlandi, M. and Marengo, E. (2018). Chemical profiling and multivariate data fusion methods for the identification of the botanical origin of honey. Food Chemistry, 266, 79-89.

Bastos, C. and Alves, R. (2003). Compostos voláteis em méis florais. Química Nova, 26, 90-96.

Belay, A., Haki, G. D., Birringer, M., Borck, H., Addi, A., Baye, K. and Melaku, S. (2017). Rheology and botanical origin of Ethiopian monofloral honey. LWT, 75, 393-401.

Belhaj, O., Oumato, J. and Zrira, S. (2015). Étude physico-chimique de quelques types de miels marocains. Revue Marocaine des Sciences Agronomiques et Vétérinaires, 3(3), 71-75.

Benaziza-Bouchema, D. and Schweitzer, P. (2010). Caractérisation des principaux miels des régions du Nord de l'Algérie. Cahiers Agricultures, 19(6), 432-438.

Beretta, G., Granata, P., Ferrero, M., Orioli, M. and Maffei Facino, R. (2005). Standardization of antioxidant properties of honey by a combination of spectrophotometric/fluorimetric assays and chemometrics. Analytica chimica acta, 533(2), 185-191.

Bogdanov, S., Lüllmann, C., Martin, P., Von Der Ohe, W., Russmann, H., Vorwohl, G., Oddo, L. P., Sabatini, A.-G., Marcazzan, G. L. and Piro, R. (1999). Honey quality and international regulatory standards: review by the International Honey Commission. Bee world, 80(2), 61-69.

Bogdanov, S., Martin, P. and Lullmann, C. (2002). Harmonised methods of the international honey commission. Swiss Bee Research Centre, FAM, Liebefeld, 5(1), 1-62.

Boutebib, A.B., N’diaye, A.D., Elhoumed, S.A.B., M’Baye, B.K., Ali, Y.A.E.H., Hammouti, B., and Semega, B.M. (2023). Assessment of iron contamination in groundwater of catchment area water. Indonesian Journal of Science and Technology, 8(3), 429-438.

Bouyahya, A., Abrini, J., Et-Touys, A., Lagrouh, F., Dakka, N. and Bakri, Y. (2018). Analyse phytochimique et évaluation de l’activité antioxydante des échantillons du miel marocain. Phytothérapie, 16(S1), 220-224.

Castro-Vázquez, L., Díaz-Maroto, M. C., De Torres, C. and Pérez-Coello, M. S. (2010). Effect of geographical origin on the chemical and sensory characteristics of chestnut honeys. Food Research International, 43(10), 2335-2340.

Castro-Vázquez, L., Leon-Ruiz, V., Alañon, M. E., Pérez-Coello, M. S. and González-Porto, A. V. (2014). Floral origin markers for authenticating Lavandin honey (Lavandula angustifolia x latifolia). Discrimination from Lavender honey (Lavandula latifolia). Food Control, 37, 362-370.

Chakir, A., Romane, A., Marcazzan, G. L. and Ferrazzi, P. 2016). Physicochemical properties of some honeys produced from different plants in Morocco. Arabian Journal of Chemistry, 9, S946-S954.

Chen, N.-N., Zhao, S.-C., Deng, L.-G., Guo, C.-Y., Mao, J.-S., Zheng, H., Yang, G.-S., Lu, X. and Aboul-Enein, H. Y. (2011). Determination of five polyphenols by HPLC/DAD and discrimination of apple varieties. Chromatographia, 73, 595-598.

Chua, L. S., Rahaman, N. L. A., Adnan, N. A. and Eddie Tan, T. T. 2013. Antioxidant Activity of Three Honey Samples in Relation with Their Biochemical Components. Journal of Analytical Methods in Chemistry, 2013.

Cianciosi, D., Forbes-Hernández, T. Y., Afrin, S., Gasparrini, M., Reboredo-Rodriguez, P., Manna, P. P., Zhang, J., Bravo Lamas, L., Martínez Flórez, S. and Agudo Toyos, P. (2018). Phenolic compounds in honey and their associated health benefits: A review. Molecules, 23(9), 2322.

Ciric, J., Sando, D., Spiric, D., Janjic, J., Boskovic, M., Glisic, M. and Baltic, M. Z. (2018). Characterization of Bosnia and Herzegovina honey according to their physico-chemical properties during 2016-2017. Scientific journal" Meat Technology", 59(1), 46-53.

Cohen, I. and Weihs, D. (2010). Rheology and microrheology of natural and reduced-calorie Israeli honeys as a model for high-viscosity Newtonian liquids. Journal of Food Engineering, 100(2), 366-371.

Conti, M. E., Finoia, M. G., Fontana, L., Mele, G., Botrè, F. and Iavicoli, I. (2014). Characterization of Argentine honeys on the basis of their mineral content and some typical quality parameters. Chemistry Central Journal, 8, 1-10.

Costa, A. C. V. D., Sousa, J. M. B., Da Silva, M. A. A. P., Garruti, D. D. S. and Madruga, M. S. (2018). Sensory and volatile profiles of monofloral honeys produced by native stingless bees of the brazilian semiarid region. Food Research International, 105, 110-120.

Dalli, M., Daoudi, N. E., Azizi, S.-E., Benouda, H., Bnouham, M. and Gseyra, N. (2021). Chemical Composition Analysis Using HPLC‐UV/GC‐MS and Inhibitory Activity of Different Nigella sativa Fractions on Pancreatic α‐Amylase and Intestinal Glucose Absorption. BioMed research international, 2021(1), 9979419.

Damto, T., Birhanu, T. and Zewdu, A. (2023). Physicochemical and antioxidant characterization of commercially available honey sample from Addis Ababa market, Ethiopia. Heliyon, 9(10), E20830.

Devi, A., Jangir, J. and K.A, A.-A. (2018). Chemical characterization complemented with chemometrics for the botanical origin identification of unifloral and multifloral honeys from India. Food Research International, 107, 216-226.

Devillers, J., Morlot, M., Pham-Delegue, M. and Dore, J. (2004). Classification of monofloral honeys based on their quality control data. Food chemistry, 86(2), 305-312.

Diass, K., Oualdi, I., Dalli, M., Azizi, S.E., Mohamed, M., Gseyra, N., Touzani, R., and Hammouti, B. (2023). Artemisia herba alba essential oil: GC/MS analysis, antioxidant activities with molecular docking on S protein of SARS-CoV-2. Indonesian Journal of Science and Technology, 8(1), 1-18

Díez, M. J., Andrés, C. and Terrab, A. (2004). Physicochemical parameters and pollen analysis of Moroccan honeydew honeys. International Journal of Food Science and Technology, 39(2), 167-176.

Doner, L. W. (1977). the Sugars of Honey—A Review. Journal of the Science of Food and Agriculture, 28, 443-456.

Duru, C. E. and Duru, I. A. (2021). Phytochemical evaluation and health risk assessment of honey from an apiary in amizi, ikuano local government area, Abia State, Nigeria. Scientific African, 13, E00885.

Ech-Chihbi, E., Hajjaji, F.E., Titi, A., Messali, M., Kaya, S., Serdaroğlu, G., Hammouti, B., and Taleb, M. (2024). Towards understanding the corrosion inhibition mechanism of green imidazolium-based ionic liquids for mild steel protection in acidic environments. Indonesian Journal of Science and Technology, 9(2), 395-420.

El Ati, R., Bouammali, H., El Kodadi, M., Touzani, R., and Hammouti, B. (2022). Study of the catecholase activity of new catalysts based on Copper (II) and heterocyclic ligands. Indonesian Journal of Science and Technology, 7(1), 1-18.

El Menyiy, N., Akdad, M., Elamine, Y. and Lyoussi, B. (2020). Microbiological quality, physicochemical properties, and antioxidant capacity of honey samples commercialized in the Moroccan Errachidia region. Journal of Food Quality, 2020, 1-9.

Erejuwa, O. O., Sulaiman, S. A. and Wahab, M. S. A. (2012). Fructose might contribute to the hypoglycemic effect of honey. Molecules, 17, 1900-1915.

Errich, A., Azzaoui, K., Mejdoubi, E., Hammouti, B., Abidi, N., Akartasse, N., Benidire, L., EL Hajjaji, S., Sabbahi, R., and Lamhamdi, A. (2021). Toxic heavy metals removal using a hydroxyapatite and hydroxyethyl cellulose modified with a new Gum Arabic. Indonesian Journal of Science and Technology, 6(1), 41-64.

Escriche, I., Visquert, M., Juan-Borrás, M. and Fito, P. (2009). Influence of simulated industrial thermal treatments on the volatile fractions of different varieties of honey. Food Chemistry, 112, 329-338.

Ferreira, I. C. F. R., Aires, E., Barreira, J. C. M. and Estevinho, L. M. (2009). Antioxidant activity of Portuguese honey samples: different contributions of the entire honey and phenolic extract. Food Chemistry, 114, 1438-1443.

Finola, M. S., Lasagno, M. C. and Marioli, J. M. (2007). Microbiological and chemical characterization of honeys from central Argentina. Food Chemistry, 100, 1649-1653.

Flieger, J. and Flieger, M. (2020). the [Dpph●/Dpph-H]-Hplc-Dad method on tracking the antioxidant activity of pure antioxidants and goutweed (Aegopodium Podagraria L.) hydroalcoholic extracts. Molecules, 25, 6005.

Gela, A., Hora, Z. A., Kebebe, D. and Gebresilassie, A. (2021). Physico-chemical characteristics of honey produced by stingless bees (Meliponula Beccaria) from west showa zone of oromia region, Ethiopia. Heliyon, 7, E05875.

Gheldof, N., Wang, X.-H. and Engeseth, N. J. (2002). Identification and quantification of antioxidant components of honeys from various floral sources. Journal of Agricultural and Food Chemistry, 50, 5870-5877.

Gomes, S., Dias, L. G., Moreira, L. L., Rodrigues, P. and Estevinho, L. (2010). Physicochemical, microbiological and antimicrobial properties of commercial honeys from Portugal. Food and Chemical Toxicology, 48, 544-548.

Hamdaoui, N., Rokni, Y., Asehraou, A., Mouncif, M., Mennane, Z., Omari, A., Sellam, A., Hammouti, B., and Meziane, M. (2023). Technological aptitude and sensitivity of lactic acid bacteria leuconostoc isolated from raw milk of cows: From step-by-step experimental procedure to the results. Indonesian Journal of Science and Technology, 8(2), 157-170.

Hameed, O. M., Shaker, O. M., Ben Slima, A. and Makni, M. (2024). Biochemical profiling and physicochemical and biological valorization of Iraqi honey: A comprehensive analysis. Molecules, 29, 671.

Hossain, M. L., Hammer, K., Lim, L. Y., Hettiarachchi, D. and Locher, C. (2022). Optimisation of an agar overlay assay for the assessment of the antimicrobial activity of topically applied semi-solid antiseptic products including honey-based formulations. Journal of Microbiological Methods, 202, 106596.

Hussein, S., Yusoff, K. M., Makpol, S. and Yusof, Y. M. (2014). Does gamma irradiation affect physicochemical properties of honey. Clinica Terapeutica, 165, 125-33.

Islam, M. K., Lawag, I. L., Green, K. J., Sostaric, T., Hammer, K. A., Lim, L. Y. and Locher, C. (2022). An investigation of the suitability of melissopalynology to authenticate jarrah honey. Current Research in Food Science, 5, 506-514.

Ismail, M., Abdallah, E. M. and Elsharkawy, E. R. (2021). Physico-chemical properties, antioxidant, and antimicrobial activity of five varieties of honey from Saudi Arabia. Asia Pacific Journal of Molecular Biology and Biotechnology, 29, 27-34.

Jarukas, L., Kuraite, G., Baranauskaite, J., Marksa, M., Bezruk, I. and Ivanauskas, L. (2020). Optimization and validation of the gc/fid method for the quantification of fatty acids in bee products. Applied Sciences, 11, 83.

Joshi, S. R., Pechhacker, H., Willam, A. and Von Der Ohe, W. (2000). Physico-chemical characteristics of apis dorsata, a. cerana and a. mellifera honey from chitwan district, central Nepal. Apidologie, 31, 367-375.

Juan-Borrás, M., Domenech, E., Hellebrandova, M. and Escriche, I. (2014). Effect of country origin on physicochemical, sugar and volatile composition of acacia, sunflower and tilia honeys. Food Research International, 60, 86-94.

Kamal, M. A. and Klein, P. (2011). Determination of sugars in honey by liquid chromatography. Saudi Journal of Biological Sciences, 18, 17-21.

Khuder, A., Ahmad, M., Hasan, R. and Saour, G. (2010). Improvement of X-Ray fluorescence sensitivity by dry ashing method for elemental analysis of bee honey. Microchemical Journal, 95, 152-157.

Küçük, M., Kolaylı, S., Karaoğlu, Ş., Ulusoy, E., Baltacı, C. and Candan, F. (2007). Biological activities and chemical composition of three honeys of different types from anatolia. Food Chemistry, 100, 526-534.

Laita, M., Hammouti, B., Sabbahi, R., Messaoudi, Z., and Benkirane, R. (2024). Effect of water regime and soil maintenance mode on vegetative growth and peach tree production. Indonesian Journal of Science and Technology, 9(1), 33-44.

Loukili, E. H., Abrigach, F., Bouhrim, M., Bnouham, M., Fauconnier, M.-L. and Ramdani, M. (2021). Chemical composition and physicochemical analysis of opuntia dillenii extracts grown in Morocco. Journal of Chemistry, 2021, 1-11.

Maarouf, F.E., Saoiabi, S., Azzaoui, K., Khalil, H., Khalil, M., El Yahyaoui, A., Saoiabi, A., Hammouti, B., Youssoufi, M.H., Shityakov, S., Hamed, O., Jodeh, S., and Sabbahi, R. (2022). Amorphous iron phosphate: Inorganic sol-gel synthesis-sodium and potassium insertion. Indonesian Journal of Science and Technology, 7(1), 187-202.

Malika, N., Mohamed, F. and Chakib, E. (2005). Microbiological and physicochemical properties of Moroccan honey. International Journal of Agriculture and Biology, 7, 773-776.

Manickavasagam, G., Saaid, M. and Lim, V. (2024). Exploring stingless bee honey from selected regions of peninsular Malaysia through gas chromatography–mass spectrometry–based untargeted metabolomics. Journal of Food Science, 89(2), 1058-1072.

Manikis, I. and Thrasivoulou, A. (2001). The relation of physicochemical characteristics of honey and the crystallization sensitive parameters. Apiacta, 36, 106-112.

Marini, F., D'aloise, A., Bucci, R., Buiarelli, F., Magrì, A. L. and Magrì, A. D. (2011). Fast analysis of 4 phenolic acids in olive oil by hplc-dad and chemometrics. Chemometrics and Intelligent Laboratory Systems, 106, 142-149.

Markelov, V. and Trushin, M. (2006). Bee venom therapy and low dose naltrexone for treatment of multiple sclerosis. Nepal Journal of Neuroscience, 3, 71-77.

Meda, A., Lamien, C., Millogo, J., Romito, M. and Nacoulma, O. (2005). Physiochemical analyses of Burkina Fasan honey. Acta Veterinaria Brno, 74, 147-152.

Mohamed, M. A., Ahmed, A. A. and Mazid, M. M. (1981). Studies on Libyan honeys. Journal of Food Quality, 4, 185-201.

Moniruzzaman, M., Khalil, M., Sulaiman, S. and Gan, S. (2012). Advances in the analytical methods for determining the antioxidant properties of honey: A review. African Journal of Traditional, Complementary and Alternative Medicines, 9, 36-42.

Nasri, H., Abdellaoui, S., Omari, A., Kada, O., Chafi, A., Hammouti, B., and Chaabane, K. (2021). Length-weight relationship and condition factor of Trachurus trachurus found in the central-east region of the Moroccan Mediterranean. Indonesian Journal of Science and Technology, 6(3), 457-468.

Ng, W. J., Ee, K. Y., Hew, P. S., Chin, H. Y. and Chin, R. (2023). Sensory, physicochemical and antioxidant properties coupled with chemometric analysis of ginger (zingiber officinale roscoe var. bentong) enriched apis cerana honey. Food Chemistry Advances, 3, 100459.

Ng, W.-J., Sit, N.-W., Ooi, P. A.-C., Ee, K.-Y. and Lim, T.-M. (2021). Botanical origin differentiation of Malaysian stingless bee honey produced by heterotrigona itama and geniotrigona thoracica using chemometrics. Molecules, 26, 7628.

Nombré, I., Schweitzer, P., Boussim, J. I. and Rasolodimby, J. M. (2010). Impacts of storage conditions on physicochemical characteristics of honey samples from Burkina Faso. African Journal of Food Science, 4, 458-463.

Nugraha, A., and Nandiyanto, A.B.D. (2021). How to read and interpret GC/MS spectra. Indonesian Journal of Multidiciplinary Research, 1(2), 171-206.

Ouahabi, S., Loukili, E. H., Daoudi, N. E., Chebaibi, M., Ramdani, M., Rahhou, I., Bnouham, M., Fauconnier, M.-L., Hammouti, B., Rhazi, L., Ayerdi Gotor, A., Dépeint, F. and Ramdani, M. (2023). Study of the phytochemical composition, antioxidant properties, and in vitro anti-diabetic efficacy of gracilaria bursa-pastoris extracts. Marine Drugs, 21, 372.

Patrignani, M., Fagúndez, G. A., Tananaki, C., Thrasyvoulou, A. and Lupano, C. E. (2018). Volatile compounds of Argentinean honeys: correlation with floral and geographical origin. Food Chemistry, 246, 32-40.

Pereira, T. C., Cruz, A. G., Guimarães, J. T., Cravotto, G. and Flores, E. M. M. (2023). Ultrasonication for honey processing and preservation: A brief overview. Food Research International, 174, 113579.

Regos, I. and Treutter, D. (2010). Optimization of a high-performance liquid chromatography method for the analysis of complex polyphenol mixtures and application for sainfoin extracts (Onobrychis Viciifolia). Journal of Chromatography A, 1217, 6169-6177.

Rikohe, I. F., Mlozi, S. H. and Ngondya, I. B. (2023). Seasons and bee foraging plant species strongly influence honey antimicrobial activity. Journal of Agriculture and Food Research, 12, 100622.

Sabbahi, R., Azzaoui, K., and Hammouti, B. (2021). An assessment of the efficacy of pheromone traps in managing the red palm weevil. Indonesian Journal of Science and Technology, 6(2), 371-384.

Sellam, A., Zerrouki, Y., Dali, M., Maleb, A., Khalid, I., Hamdaoui, N., Hammouti, B., and Meziane, M. (2024). In vitro antioxidant properties of lactobacillus gasseri isolated from fermented milk: Isolation and preparation of strains and intracellular cell-free extracts, chemical testing (hydrogen peroxide, superoxide, hydroxyl radical, DPPH, ferrous ion chelating, and linoleic acid peroxidation). Indonesian Journal of Science and Technology, 9(3), 611-622.

Smanalieva, J. and Senge, B. 2009. Analytical and rheological investigations into selected unifloral German honey. European Food Research and Technology, 229, 107-113.

Socha, R., Juszczak, L., Pietrzyk, S. and Fortuna, T. (2009). Antioxidant activity and phenolic composition of herbhoneys. Food Chemistry, 113, 568-574.

Soria, A. C., Martínez-Castro, I. and Sanz, J. (2008). Some aspects of dynamic headspace analysis of volatile components in honey. Food Research International, 41, 838-848.

Stanimirova, I., Üstün, B., Cajka, T., Riddelova, K., Hajslova, J., Buydens, L. M. C. and Walczak, B. (2010). Tracing the geographical origin of honeys based on volatile compounds profiles assessment using pattern recognition techniques. Food Chemistry, 118, 171-176.

Stihi, C., Chelarescu, E. D., Duliu, O. and Toma, L. (2016). Characterization of Romanian honey using physico-chemical parameters and the elemental content determined by analytical techniques. Romanian Reports in Physics, 68, 362-369.

Subagyono, R.D.J., Qi, Y., Chaffee, A.L., Amirta, R., and Marshall, M. (2021). Pyrolysis-GC/MS analysis of fast growing wood macaranga species. Indonesian Journal of Science and Technology, 6(1), 141-158.

Terrab, A., Gonzalez, A. G., Díez, M. J. and Heredia, F. J. (2003). Mineral content and electrical conductivity of the honeys produced in northwest Morocco and their contribution to the characterisation of unifloral honeys. Journal of the Science of Food and Agriculture, 83, 637-643.

Terzo, S., Mulè, F. and Amato, A. (2020). Honey and obesity-related dysfunctions: A summary on health benefits. The Journal of Nutritional Biochemistry, 82, 108401.

Tomczyk, M., Zaguła, G. and Dżugan, M. (2020). A simple method of enrichment of honey powder with phytochemicals and its potential application in isotonic drink industry. Food Science and Technology, 125, 109204.

Trisha, S., Mortuza, M. G., Rana, J., Islam, K. H., Ferdoush, Z., Antora, R. A., Akash, S. I., Aziz, M. G. and Uddin, M. B. (2023). Evaluation of the physicochemical qualities and antioxidant properties of some bangladeshi varieties of honey: A comparative study. Journal of Agriculture and Food Research, 14, 100837.

Yamani, H. A. and Ali, W. M. (2023). Medicinal plant (ocimum basilicum) based enhancement of honey bioactivity. South African Journal of Botany, 163, 427-432.

Young, G.-W. Z. and Blundell, R. (2023). A review on the phytochemical composition and health applications of honey. Heliyon, 9(2), e12507.

Zapata-Vahos, I. C., Henao-Rojas, J. C., Yepes-Betancur, D. P., Marín-Henao, D., Giraldo Sánchez, C. E., Calvo-Cardona, S. J., David, D. and Quijano-Abril, M. (2023). Physicochemical parameters, antioxidant capacity, and antimicrobial activity of honeys from tropical forests of Colombia: Apis mellifera and Melipona eburnea. Foods, 12, 1001.

Zhang, X.-H., Qing, X.-D., Zheng, J.-J., Yu, Y., Huang, J., Kang, C. and Liu, Z. (2023). Aqueous two-phase systems coupled with chemometrics-enhanced HPLC-dad for simultaneous extraction and determination of flavonoids in honey. Food Chemistry, 19, 100766.

Zhang, X.-H., Wu, H.-L., Wang, J.-Y., Tu, D.-Z., Kang, C., Zhao, J., Chen, Y., Miu, X.-X. and Yu, R.-Q. (2013). fast HPLC-dad quantification of nine polyphenols in honey by using second-order calibration method based on trilinear decomposition algorithm. Food Chemistry, 138, 62-69.




DOI: https://doi.org/10.17509/ajse.v4i3.75415

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