Email this article 
Isolation and Identification of Hydrocarbon Degradation Bacteria and Phosphate Solubilizing Bacteria in Oil Contaminated Soil in Bojonegoro, East Java, Indonesia
Abstract
Petroleum is a mixture of hydrocarbon complexes with organic compounds from sulfur, oxygen, nitrogen and metal-containing compounds. These organic compounds can be used as substrate for bacterial growth. This study aimed to isolate and identify hydrocarbon degrading bacteria and phosphate solubilizing bacteria in oil-contaminated soil in Bojonegoro. This study used an exploration method to find each of the two types of hydrocarbon degrading bacteria and phosphate solubilizing bacteria from soil samples in Bojonegoro that contaminated by oil. Identification of isolates bacterial included macroscopic observations of bacteria, gram staining on bacterial cells and physiological tests. Macroscopic observations include the form of colonies, colony diameter, colony color, colony edge, and elevation. The physiological test using Microbact Identification System to determine the physiological characteristics of bacteria so that genera and types of bacteria can be known. The identification of organisms was based on changes in pH and use of the substrate. The results of data analysis were obtained from five types of bacteria from soil samples that contaminated by oil which were successfully isolated. After identification of species was done, four species of bacteria were obtained, namely Pseudomonas pseudomallei, Pseudomonas fluorescens-25, Flavobacterium odoratum, and Enterococcus sp.
Keywords
Full Text:
PDFReferences
Atlas, R.M. (1975). Effects of temperature and crude oil composition on petroleum biodegradation. Journal of Applied Microbiology, 30(3), 396–403.
Beveridge, T.J. (2001). Use of the Gram stain in microbiology. Biotechnic and Histochemistry, 76(3), 111-118.
Bhattacharya, D., Sarma, P.M., Krishnan, S., Mishra, S., and Lal, B. (2003) Evaluation of genetic diversity among Pseudomonas citronellolis strains isolated from oily sludgecontaminated sites. Applied Environmental Microbiology, 69(3), 1435–1441.
Borah, D., and Yadav, R.N.S. (2016). Bioremediation of petroleum based contaminants with biosurfactan produced by a newly isolated petroleum oil degrading bacterial strain. Egyptian Journal of Petroleum, 10, 1-8.
Das, N., and Chandran, P. (2011). Microbial degradation of petroleum hydrocarbon contaminants: An overview. Biotechnology Research International, 2011, 1-13.
Hamamura, N., Olson, S.H., Ward, D.M., and Inskeep, W.P. (2006). Microbial population dynamics associated with crude-oil biodegradation in diverse soils. Applied Environmental Microbiology, 72(9), 6316–6324.
Harayama, S., Kishira, H., Kasai, Y., and Shutsubo, K. (1999). Petroleum biodegradation in marine environments. Journal of Microbial Biotechnology, 1(1), 63-70.
Haritash, A.K., and Kaushik, C.P. (2009). Biodegradation aspects of polycyclic aromatic hydrocarbons (PAHs): A review. Journal of Hazardous Materials, 169, 1-15.
Holst, M.T., Wentzel, A., Ellingsen, T.E., Kotlar, H.K., and Zotchev, S.B. (2007). Identification of novel genes involved in long-chain n-alkane degradation by Acinetobacter sp. Strain DSM 17874. Applied and Environmental Microbiology, 73(10), 3327–3332.
Jain, P.K., Gupta, V.K., Gaur, R.K., Lowry, M., Jaroly, D.P., and Chauhan, U.K. (2011). Bioremediation of petroleum oil contaminated soil and water. Research Journal of Environment Toxicology, 5(1), 1-26
Kabirun, S. (2004). Peranan mikoriza arbuskular pada pertanian berkelanjutan. pidato pengukuhan jabatan guru besar dalam ilmu mikrobiologi pada fakultas pertanian UGM. Yogyakarta.
Kafilzadeh, F., Sahragard, P., Jamali, H., and Tahery Y. (2011). Isolation and identification of hydrocarbons degrading bacteria in soil around Shiraz Refinery. African Journal of Microbiology Research, 4(19), 3084-3089.
Karigar, C.S., and Rao, S.S. (2011). Role of microbial enzymes in the bioremediation of pollutants: A review. Enzyme Research, 2011, 1-11.
Liu, Y., and Kujawinski, E. B. (2015). Chemical composition and potential environmental impacts of water-soluble polar crude oil components inferred from ESI FT-ICR MS. PloS One, 10(9), e0136376.
Madihah, M. S., Ariff, A. B., Akmam, F. H., Baharuddin, A. G., and Karim, M. I. A. (1998). Hyper-thermophilic fermentative bacteria in Malaysian petroleum reservoirs. Asia-Pacific Journal of Molecular Biology and Biotechnology, 6(1), 29-37.
Millioli, V.S., Servulo, E-L.C., Sobral, L.G.S., dan Carvalho, D.D.DE. (2009). Bioremediation of crude oil-bearing soil: evaluating the effect of rhamnolipid addition to soil toxicity and to crude oil biodegradation efficiency. Global NEST Journal, 11(2), 181-188.
Mrozik, A., Piotrowska-Seget, Z., and Labuzek, S. (2003) Bacterial degradation and bioremediation of polycyclic aromatic hydrocarbons. Polish Journal of Environmental Studies, 12 (1) 15-25.
Murínová, S., and Dercová, K. (2014). Response mechanisms of bacterial degraders to environmental contaminants on the level of cell walls and cytoplasmic membrane. International Journal of Microbiology, 2014, 1-16.
Nandiyanto, A.B.D., Halimatul, H.S., Rosyid, N.H., and Effendi, D. B. (2016). Effect of tungsten concentration on growth of acetobacter xylinum as a promising agent for eco-friendly recycling system. IOP Conference Series: Materials Science and Engineering, 128 (1), 012041.
Ogbo, E.M., and Okhouya, J.A. (2011). Bioavailability of Some Heavy Metals in Crude Oil Contamined Soils Remediated with Pleurotus tuber-regium Fr. Singer. Asian Journal of Biological Sciences, 4(1), 53-61.
Parthipan, P., Preetham, E., Machuca, L.L., Pattanathu, K. S.M., Murugan, K.R., and Rajasekar, A. (2017). Biosurfactant and degradative enzymes mediated crude oil degradation by bacterium bacillussubtilis A1. Frontier Microbiology, 8, 193-197.
Rathi, M., and Gaur, N. (2016). Phosphate soluzibilizing bacteria as biofertilizer and its applications. Journal of Pharmacy Research, 10(3), 146-148.
Rodrigues, E.M., Karlos, H.M., and Marcos, R.T. (2015). Prospect, isolation, and characterization of microorganisms for potential use in cases of oil bioremediation along the coast of Trindade Island Brazil. Journal of Environmental Management, 156, 15-22.
Romanus, A.A., Omolola, A.E., Patrick, A.S., and Ifeoma, O.G. (2015). Bacterial degradation of petroleum hydrocarbons in crude oil polluted soil amended with cassava peels. American Journal of Research Communication, 3(7), 99-118.
Santhini, K., Myla, J., Sajani, S., dan Usharani, G. (2009). Screening of micrococcus sp from oil contaminated soil with reference to bioremediation. Botany Research International, 2(4), 248-252.
Sood, N., Patle, S., and Lal, B. (2010). Bioremediation of acidic oily sludge-contaminated soil by the novel yeast strain Candida digboiensis TERI ASN6. Environmental Science Pollution Research, 17(3), 603–10.
Van Hamme, J.D., Singh, A., and Ward, O.P. (2003). Recent advances in petroleum microbiology. Microbiology Molecular Biology Review, 67(4), 503–549.
Viñas, M., Sabaté, J., Espuny, M.J., and Solanas, A.M. (2005). Bacterial community dynamics and polycyclic aromatic hydrocarbon degradation during bioremediation of heavily creosote-contaminated soil. Applied Environmental Microbiology, 71(11), 7008–7018.
Xenia, M.E., and Refugio, R.V. (2016). Microorganisms metabolism during bioremediation of oil contaminated soils. Journal of Bioremediation and Biodegradation, 7(2), 20-25.
DOI: https://doi.org/10.17509/ijost.v4i1.14923
Refbacks
- There are currently no refbacks.
Copyright (c) 2019 Indonesian Journal of Science and Technology
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Indonesian Journal of Science and Technology is published by UPI.
View My Stats